Anti-Recombinant-HK1 antibody [RM1RM18] (STJA0024101)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Hexokinase-1 for use in IF, IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM1RM18 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200 |
| Formulation | 0.01M PBS |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | HK1 |
| Gene ID | 3098 |
| Uniprot ID | HXK1_HUMAN |
Additional Info
| Function | Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome. |
| Protein Name | Hexokinase-1Brain Form HexokinaseHexokinase Type IHk IHexokinase-A |
| Database Links | Reactome: R-HSA-446205Reactome: R-HSA-5619056Reactome: R-HSA-70171 |
| Cellular Localisation | Mitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmCytosolThe Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-GlucosamineLeading To Relocation To The Cytosol |
| Alternative Antibody Names | Anti-Hexokinase-1 antibodyAnti-Brain Form Hexokinase antibodyAnti-Hexokinase Type I antibodyAnti-Hk I antibodyAnti-Hexokinase-A antibodyAnti-HK1 antibody |
Information sourced from Uniprot.org