Anti-Recombinant-GNA13 antibody [RM2F40] (STJA0025509)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Guanine Nucleotide-Binding Protein Subunit Alpha-13 for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2F40 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IHC: 1:50-1:100, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | GNA13 |
| Gene ID | 10672 |
| Uniprot ID | GNA13_HUMAN |
Additional Info
| Post Translational Modifications | Palmitoylation is critical for proper membrane localization and signaling. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation. |
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in a process independent from Rho activation. In lymphoid follicles, transmits P2RY8- and S1PR2-dependent signals that lead to inhibition of germinal center (GC) B cell growth and migration outside the GC niche. |
| Protein Name | Guanine Nucleotide-Binding Protein Subunit Alpha-13G Alpha-13G-Protein Subunit Alpha-13 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-428930Reactome: R-HSA-456926Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Cell MembraneLipid-AnchorMelanosomeCytoplasmNucleusIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvDetected In The Cytoplasm Of Leydig Cells And In The Seminiferous EpitheliumIncluding Differentiating Cells From The Spermatogonia To Mature Spermatozoa StagesIn Round SpermatidsAlso Present In The Nuclei |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-13 antibodyAnti-G Alpha-13 antibodyAnti-G-Protein Subunit Alpha-13 antibodyAnti-GNA13 antibody |
Information sourced from Uniprot.org