Anti-Recombinant-ELP3 antibody [RM2N15] (STJA0026177)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Elongator Complex Protein 3 for use in IF, IP and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2N15 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | ELP3 |
| Gene ID | 55140 |
| Uniprot ID | ELP3_HUMAN |
Additional Info
| Post Translational Modifications | Tyrosine-phosphorylated.phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability. Also serine/threonine-phosphorylated. |
| Function | Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins.such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex. |
| Protein Name | Elongator Complex Protein 3Help3Trna Uridine(34 Acetyltransferase |
| Database Links | Reactome: R-HSA-3214847 |
| Cellular Localisation | CytoplasmNucleusIsoform 1: NucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Elongator Complex Protein 3 antibodyAnti-Help3 antibodyAnti-Trna Uridine(34 Acetyltransferase antibodyAnti-ELP3 antibody |
Information sourced from Uniprot.org