Anti-Recombinant-DROSHA antibody [RM2K44] (STJA0025909)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Ribonuclease 3 for use in FC, IF, IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2K44 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | DROSHA |
| Gene ID | 29102 |
| Uniprot ID | RNC_HUMAN |
Additional Info
| Post Translational Modifications | Degraded by autophagy in response to neuronal activity in motor neurons. |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons. |
| Protein Name | Ribonuclease 3Protein DroshaRibonuclease IiiRnase IiiP241 |
| Database Links | Reactome: R-HSA-203927 |
| Cellular Localisation | NucleusNucleolusCytoplasmA Fraction Is Translocated To The Nucleolus During The S Phase Of The Cell CycleLocalized In Gw Bodies (Gwbs)Also Known As P-Bodies |
| Alternative Antibody Names | Anti-Ribonuclease 3 antibodyAnti-Protein Drosha antibodyAnti-Ribonuclease Iii antibodyAnti-Rnase Iii antibodyAnti-P241 antibodyAnti-DROSHA antibodyAnti-RN3 antibodyAnti-RNASE3L antibodyAnti-RNASEN antibody |
Information sourced from Uniprot.org