Anti-Recombinant-DIABLO antibody [RM2M81] (STJA0026144)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Diablo Iap-Binding Mitochondrial Protein for use in IF, IHC, IP and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2M81 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | DIABLO |
| Gene ID | 56616 |
| Uniprot ID | DBLOH_HUMAN |
Additional Info
| Post Translational Modifications | Ubiquitinated by BIRC7/livin. Ubiquitinated by BIRC6. The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells. |
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases. Isoform 3: Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1: Defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
| Protein Name | Diablo Iap-Binding Mitochondrial ProteinDiablo Homolog - MitochondrialDirect Iap-Binding Protein With Low PiSecond Mitochondria-Derived Activator Of CaspasesSmac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111469Reactome: R-HSA-9627069 |
| Cellular Localisation | MitochondrionCytoplasmCytosolReleased Into The Cytosol In A Parl-Dependent Manner When Cells Undergo Apoptosis |
| Alternative Antibody Names | Anti-Diablo Iap-Binding Mitochondrial Protein antibodyAnti-Diablo Homolog - Mitochondrial antibodyAnti-Direct Iap-Binding Protein With Low Pi antibodyAnti-Second Mitochondria-Derived Activator Of Caspases antibodyAnti-Smac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form antibodyAnti-DIABLO antibodyAnti-SMAC antibody |
Information sourced from Uniprot.org