| Short Description : | Rabbit monoclonal anti-Recombinant-Mitotic Checkpoint Protein Bub3 for use in FC, IF, IHC, IP and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | FC/IF/IHC/IP/WB |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | RM1E28 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Protein A/G purified from cell culture supernatant |
| Dilution Range: | FCM: 1:20-1:100 |
| Formulation: | 0.01M PBS |
| Storage Instruction: | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Protein Name | Mitotic Checkpoint Protein Bub3 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreStarts To Localize At Kinetochores In Prometaphase I (Pro-Mi) Stage And Maintains The Localization Until The Metaphase I-Anaphase I (Mi-Ai) Transition |
| Alternative Antibody Names | Anti-Mitotic Checkpoint Protein Bub3 antibodyAnti-BUB3 antibody |
Information sourced from Uniprot.org
