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Anti-Recombinant-beta Actin antibody [SR4971] (STJA0004971)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide corresponding to Mouse beta-Actin
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-beta Actin for use in WB, IHC, IF and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SR4971 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB (H, M, R) 1:1000-1:2000IHC/IF (H, M, R) 1:500-1:1000ICC/IF (H, M, R) 1:300-1:1000 |
| Formulation | PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | Actb |
| Gene ID | 11461 |
| Uniprot ID | ACTB_MOUSE |
| Immunogen | KLH conjugated synthetic peptide corresponding to Mouse beta-Actin |
Additional Info
| Post Translational Modifications | ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery. Actin, cytoplasmic 1: N-terminal cleavage of acetylated methionine of immature cytoplasmic actin by ACTMAP. Actin, cytoplasmic 1, N-terminally processed: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected. |
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules. |
| Protein Name | Actin - Cytoplasmic 1Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed |
| Database Links | Reactome: R-MMU-190873Reactome: -MMU-196025Reactome: -MMU-2029482Reactome: -MMU-3928662Reactome: -MMU-418990Reactome: -MMU-437239Reactome: -MMU-4420097Reactome: -MMU-445095Reactome: -MMU-446353Reactome: -MMU-5250924Reactome: -MMU-5626467Reactome: -MMU-5663213Reactome: -MMU-5663220Reactome: -MMU-5674135Reactome: -MMU-5689603Reactome: -MMU-5696394Reactome: -MMU-8856828Reactome: -MMU-9035034Reactome: -MMU-9913351 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative Antibody Names | Anti-Actin - Cytoplasmic 1 antibodyAnti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibodyAnti-Actb antibody |
Information sourced from Uniprot.org