Anti-Recombinant-Bassoon antibody (157-517 aa) [L124/59] (STJA0040516)
SPECIFICATIONS
ClonalityMonoclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenFusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli
General Information
| Short Description | Chicken monoclonal anti-Recombinant-Bassoon (157-517 aa) for use in WB, IHC, ICC, ELISA and AT in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC/ELISA/AT |
| Host | Chicken |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | L124/59 |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by affinity chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:100ICC 1:100 |
| Formulation | 1X PBS, 0.05% Sodium Azide pH 7.4 |
| Storage Instruction | Aliquot and store at ≤-20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
Target Information
| Gene Symbol | BsnUniprot ID=BSN_RAT" |
| Immunogen | Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli |
| Immunogen Region | 157-517 aa |
| Immunogen Sequence | Rat |
| Specificity | No cross-reactivity reported |
Additional Info
| Post Translational Modifications | Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization. |
| Function | Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released. After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts. At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis. Also functions in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5. Also mediates synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import. Inhibits the activity of the proportion of DAO enzyme that localizes to the presynaptic active zone, which may modulate synaptic transmission. |
| Protein Name | Protein Bassoon |
| Cellular Localisation | CytoplasmPresynaptic Active ZoneCytoskeletonCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembranePeripheral Membrane ProteinIn RetinaIs Localized In The Outer Plexiform Layer At Ribbon Synapses Formed By Rods And Cones But Was Absent From Basal Synaptic Contacts Formed By ConesIn The Retinal Inner Plexiform Layer Localized To Conventional Inhibitory Gabaergic SynapsesMade By Amacrine CellsBut Absent From The Bipolar Cell Ribbon Synapses |
| Alternative Antibody Names | Anti-Protein Bassoon antibodyAnti-Bsn antibody |
Information sourced from Uniprot.org