Anti-RECK antibody (110-210) (STJ28801)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RECK (110-210) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RECK |
| Gene ID | 8434 |
| Uniprot ID | RECK_HUMAN |
| Immunogen Region | 110-210 |
| Immunogen Sequence | ALECRQACKQASSKNDISKV CRKEYENALFSCISRNEMGS VCCSYAGHHTNCREYCQAIF RTDSSPGPSQIKAVENYCAS ISPQLIHCVNNYTQSYPMRN P |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 110-210 of human RECK (NP_066934.1). |
Additional Info
| Tissue Specificity | Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells. |
| Post Translational Modifications | N-glycosylated. |
| Function | Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7. ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity. Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP). |
| Protein Name | Reversion-Inducing Cysteine-Rich Protein With Kazal MotifsHreckSuppressor Of Tumorigenicity 15 Protein |
| Database Links | Reactome: R-HSA-163125 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-Reversion-Inducing Cysteine-Rich Protein With Kazal Motifs antibodyAnti-Hreck antibodyAnti-Suppressor Of Tumorigenicity 15 Protein antibodyAnti-RECK antibodyAnti-ST15 antibody |
Information sourced from Uniprot.org