• Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using CoREST/RCOR1 antibody (STJ114711) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g CoREST/RCOR1 antibody (STJ114711). Western blot was performed from the immunoprecipitate using CoREST/RCOR1 antibody (STJ114711) at a dilution of 1:1000.
  • Western blot analysis of various lysates using CoREST/RCOR1 Rabbit polyclonal antibody (STJ114711) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RCOR1 antibody (256-485) (STJ114711)

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STJ114711

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RCOR1 (256-485) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RCOR1
Gene ID: 23186
Uniprot ID: RCOR1_HUMAN
Immunogen Region: 256-485
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 256-485 of human CoREST/CoREST/RCOR1 (NP_055971.2).
Immunogen Sequence: EREESEDELEEANGNNPIDI EVDQNKESKKEVPPTETVPQ VKKEKHSTQAKNRAKRKPPK GMFLSQEDVEAVSANATAAT TVLRQLDMELVSVKRQIQNI KQTNSALKEKLDGGIEPYRL PEVIQKCNARWTTEEQLLAV QAIRKYGRDFQAISDVIGNK SVVQVKNFFVNYRRRFNIDE VLQEWEAEHGKEETNGPSNQ KPVKSPDNSIKMPEEEDEAP VLDVRYASAS
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by HSV-1 protein kinases in case of infection.
Function Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.
Protein Name Rest Corepressor 1
Protein Corest
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Upon Infection By Hsv-1
It Is Partially Translocated Into The Cytoplasm In An Hsv-1-Dependent Manner
Alternative Antibody Names Anti-Rest Corepressor 1 antibody
Anti-Protein Corest antibody
Anti-RCOR1 antibody
Anti-KIAA0071 antibody
Anti-RCOR antibody

Information sourced from Uniprot.org

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