• Immunohistochemistry analysis of RCE1 in paraffin-embedded Human esophageal using RCE1 Rabbit polyclonal antibody (STJ11100368) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RCE1 Rabbit polyclonal antibody (STJ11100368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RCE1 antibody (100-200) (STJ11100368)

SKU:
STJ11100368

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RCE1 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RCE1
Gene ID: 9986
Uniprot ID: FACE2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RCE1 (NP_005124.1).
Immunogen Sequence: SLLTLMGFRLEGIFPAALLP LLLTMILFLGPLMQLSMDCP CDLADGLKVVLAPRSWARCL TDMRWLRNQVIAPLTEELVF RACMLPMLAPCMGLGPAVFT C
Tissue Specificity Ubiquitous.
Post Translational Modifications Ubiquitinated. Undergoes 'Lys-48'- and 'Lys-63'-linked ubiquitination. 'Lys-48' ubiquitination induces its degradation. Deubiquitinated by USP17L2/USP17 that cleaves 'Lys-63'-linked ubiquitin chains.
Function Proteolytically removes the C-terminal three residues of farnesylated and geranylated proteins. Seems to be able to process K-Ras, N-Ras, H-Ras, RAP1B and G-gamma-1.
Protein Name Caax Prenyl Protease 2
Farnesylated Proteins-Converting Enzyme 2
Face-2
Prenyl Protein-Specific Endoprotease 2
Rce1 Homolog
Hrce1
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-9648002
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Caax Prenyl Protease 2 antibody
Anti-Farnesylated Proteins-Converting Enzyme 2 antibody
Anti-Face-2 antibody
Anti-Prenyl Protein-Specific Endoprotease 2 antibody
Anti-Rce1 Homolog antibody
Anti-Hrce1 antibody
Anti-RCE1 antibody
Anti-FACE2 antibody
Anti-RCE1A antibody
Anti-RCE1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance