• Western blot analysis of lysates from wild type (WT) and RBM3 knockout (KO) 293T cells, using [KO Validated] RBM3 Rabbit polyclonal antibody (STJ11100843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RBM3 antibody (1-157) (STJ11100843)

SKU:
STJ11100843

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBM3 (1-157) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBM3
Gene ID: 5935
Uniprot ID: RBM3_HUMAN
Immunogen Region: 1-157
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human RBM3 (NP_006734.1).
Immunogen Sequence: MSSEEGKLFVGGLNFNTDEQ ALEDHFSSFGPISEVVVVKD RETQRSRGFGFITFTNPEHA SVAMRAMNGESLDGRQIRVD HAGKSARGTRGGGFGAHGRG RSYSRGGGDQGYGSGRYYDS RPGGYGYGYGRSRDYNGRNQ GGYDRYSGGNYRDNYDN
Post Translational Modifications Arg-105 is dimethylated, probably to asymmetric dimethylarginine. Phosphorylated.
Function Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.
Protein Name Rna-Binding Protein 3
Rna-Binding Motif Protein 3
Rnpl
Cellular Localisation Nucleus
Cytoplasm
Cell Projection
Dendrite
Localizes In Mrna Granules In Dentrites
Alternative Antibody Names Anti-Rna-Binding Protein 3 antibody
Anti-Rna-Binding Motif Protein 3 antibody
Anti-Rnpl antibody
Anti-RBM3 antibody
Anti-RNPL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance