![Western blot analysis of lysates from wild type (WT) and RBM3 knockout (KO) 293T cells, using [KO Validated] RBM3 Rabbit polyclonal antibody (STJ11100843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89777/358248/STJ11100843_1__06496.1713122583.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RBM3 knockout (KO) 293T cells, using [KO Validated] RBM3 Rabbit polyclonal antibody (STJ11100843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western blot analysis of lysates from wild type (WT) and RBM3 knockout (KO) 293T cells, using [KO Validated] RBM3 Rabbit polyclonal antibody (STJ11100843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89777/358248/STJ11100843_1__06496.1713122583.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RBM3 knockout (KO) 293T cells, using [KO Validated] RBM3 Rabbit polyclonal antibody (STJ11100843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
Anti-RBM3 antibody (1-157) (STJ11100843)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBM3 (1-157) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBM3 |
| Gene ID | 5935 |
| Uniprot ID | RBM3_HUMAN |
| Immunogen Region | 1-157 |
| Immunogen Sequence | MSSEEGKLFVGGLNFNTDEQ ALEDHFSSFGPISEVVVVKD RETQRSRGFGFITFTNPEHA SVAMRAMNGESLDGRQIRVD HAGKSARGTRGGGFGAHGRG RSYSRGGGDQGYGSGRYYDS RPGGYGYGYGRSRDYNGRNQ GGYDRYSGGNYRDNYDN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human RBM3 (NP_006734.1). |
Additional Info
| Post Translational Modifications | Arg-105 is dimethylated, probably to asymmetric dimethylarginine. Phosphorylated. |
| Function | Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation. |
| Protein Name | Rna-Binding Protein 3Rna-Binding Motif Protein 3Rnpl |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCell ProjectionDendriteLocalizes In Mrna Granules In Dentrites |
| Alternative Antibody Names | Anti-Rna-Binding Protein 3 antibodyAnti-Rna-Binding Motif Protein 3 antibodyAnti-Rnpl antibodyAnti-RBM3 antibodyAnti-RNPL antibody |
Information sourced from Uniprot.org