• Western blot analysis of lysates from Raji cells, using RBM19 Rabbit polyclonal antibody (STJ119666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-RBM19 antibody (759-960) (STJ119666)

SKU:
STJ119666

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBM19 (759-960) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBM19
Gene ID: 9904
Uniprot ID: RBM19_HUMAN
Immunogen Region: 759-960
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 759-960 of human RBM19 (NP_057280.2).
Immunogen Sequence: CSISKKKNKAGVLLSMGFGF VEYRKPEQAQKALKQLQGHV VDGHKLEVRISERATKPAVT LARKKQVPRKQTTSKILVRN IPFQAHSREIRELFSTFGEL KTVRLPKKMTGTGTHRGFGF VDFLTKQDAKRAFNALCHST HLYGRRLVLEWADSEVTLQA LRRKTAAHFHEPPKKKRSVV LDEILEQLEGSDSDSEEQTL QL
Tissue Specificity Expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia (at protein level).
Function Plays a role in embryo pre-implantation development.
Protein Name Probable Rna-Binding Protein 19
Rna-Binding Motif Protein 19
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Cytoplasm
Chromosome
In Discrete Foci Distributed Throughout The Cytoplasm And Nucleoplasm During The 4 To 8 Cell Stages And The Morula Stage
But Not In The Periphery Of The Nucleolar Precursor Body (Npb)
During Blastocyst Development
Becomes Increasingly Localized To The Nucleolus And Less To The Cytoplasm
At The Late Blastocyst Stage
Localized Predominantly In The Nucleolus
Localized In The Nucleolus During Interphase And To The Perichromosomal Sheath During Mitosis
Does Not Colocalize In The Cytoplasm With Gw182 In P-Bodies
May Translocate To The Nucleolus Upon Early Embryonic Development
Colocalizes With Npm1 During Interphase
By Late Prophase
Metaphase
Anaphase And Telophase
Associates With The Chromosome Periphery
By Telophase Localizes To Npb
Alternative Antibody Names Anti-Probable Rna-Binding Protein 19 antibody
Anti-Rna-Binding Motif Protein 19 antibody
Anti-RBM19 antibody
Anti-KIAA0682 antibody

Information sourced from Uniprot.org

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