• Immunohistochemistry analysis of paraffin-embedded mouse spleen using RBM17 antibody (STJ26216) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human endometrium cancer using RBM17 antibody (STJ26216) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using RBM17 antibody (STJ26216) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RBM17 antibody (STJ26216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RBM17 antibody (1-240) (STJ26216)

SKU:
STJ26216

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBM17 (1-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBM17
Gene ID: 84991
Uniprot ID: SPF45_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1).
Immunogen Sequence: MSLYDDLGVETSDSKTEGWS KNFKLLQSQLQVKKAALTQA KSQRTKQSTVLAPVIDLKRG GSSDDRQIVDTPPHVAAGLK DPVPSGFSAGEVLIPLADEY DPMFPNDYEKVVKRQREERQ RQRELERQKEIEEREKRRKD RHEASGFARRPDPDSDEDED YERERRKRSMGGAAIAPPTS LVEKDKELPRDFPYEEDSRP RSQSSKAAIPPPVYEEQDRP RSPTGPSNSFLANMGGTVA
Function Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Protein Name Splicing Factor 45
45 Kda-Splicing Factor
Rna-Binding Motif Protein 17
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 45 antibody
Anti-45 Kda-Splicing Factor antibody
Anti-Rna-Binding Motif Protein 17 antibody
Anti-RBM17 antibody
Anti-SPF45 antibody

Information sourced from Uniprot.org

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