| Short Description : | Rabbit monoclonal NeuN (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S3MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:6000IHC-P: 1:500-1:2000IF/ICC:1:200-1:800ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RBFOX3 |
| Gene ID: | 146713 |
| Uniprot ID: | RFOX3_HUMAN |
| Immunogen Region: | 1-100 |
| Immunogen Sequence: | MAQPYPPAQYPPPPQNGIPA EYAPPPPHPTQDYSGQTPVP TEHGMTLYTPAQTHPEQPGS EASTQPIAGTQTVPQTDEAA QTDSQPLHPSDPTEKQQPKR |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NeuN (A6NFN3). |
| Function | Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). |
| Protein Name | Rna Binding Protein Fox-1 Homolog 3Fox-1 Homolog CNeuronal Nuclei AntigenNeun Antigen |
| Database Links | Reactome: R-HSA-9768919 |
| Cellular Localisation | NucleusCytoplasmLargely Restricted To Neuronal NucleiHoweverSignificant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment |
| Alternative Antibody Names | Anti-Rna Binding Protein Fox-1 Homolog 3 antibodyAnti-Fox-1 Homolog C antibodyAnti-Neuronal Nuclei Antigen antibodyAnti-Neun Antigen antibodyAnti-RBFOX3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cell pools, using [KD Validated] GPX4 Rabbit monoclonal antibody (STJ11101303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90216/359234/STJ11101303_1__60787.1713123659.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cell pools, using [KD Validated] GPX4 Rabbit monoclonal antibody (STJ11101303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
