• Western blot analysis of various lysates, using RBFOX1 antibody (STJ110121) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of mouse brain cells using RBFOX1 rabbit polyclonal antibody (STJ110121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using RBFOX1 rabbit polyclonal antibody (STJ110121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RBFOX1 antibody (1-130) (STJ110121)

SKU:
STJ110121

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBFOX1 (1-130) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBFOX1
Gene ID: 54715
Uniprot ID: RFOX1_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBFOX1 (NP_665900.1).
Immunogen Sequence: MLASQGVLLHPYGVPMIVPA APYLPGLIQGNQEAAAAPDT MAQPYASAQFAPPQNGIPAE YTAPHPHPAPEYTGQTTVPE HTLNLYPPAQTHSEQSPADT SAQTVSGTATQTDDAAPTDG QPQTQPSENT
Tissue Specificity Predominantly expressed in muscle and brain.
Function RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
Protein Name Rna Binding Protein Fox-1 Homolog 1
Ataxin-2-Binding Protein 1
Fox-1 Homolog A
Hexaribonucleotide-Binding Protein 1
Database Links Reactome: R-HSA-9022707
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 1 antibody
Anti-Ataxin-2-Binding Protein 1 antibody
Anti-Fox-1 Homolog A antibody
Anti-Hexaribonucleotide-Binding Protein 1 antibody
Anti-RBFOX1 antibody
Anti-A2BP antibody
Anti-A2BP1 antibody
Anti-FOX1 antibody
Anti-HRNBP1 antibody

Information sourced from Uniprot.org

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