Anti-RBCK1 antibody (60-192) (STJ11103321)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBCK1 (60-192) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBCK1 |
| Gene ID | 10616 |
| Uniprot ID | HOIL1_HUMAN |
| Immunogen Region | 60-192 |
| Immunogen Sequence | SVEDAQMHTVTIWLTVRPDM TVASLKDMVFLDYGFPPVLQ QWVIGQRLARDQETLHSHGV RQNGDSAYLYLLSARNTSLN PQELQRERQLRMLEDLGFKD LTLQPRGPLEPGPPKPGVPQ EPGRGQPDAVPEP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 60-192 of human RBCK1 (NP_112506.2). |
Additional Info
| Post Translational Modifications | Auto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome. Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity. (Microbial infection) Ubiquitinated by S.flexneri E3 ubiquitin-protein ligases IpaH1.4 and IpaH2.5, leading to its degradation by the proteasome, thereby preventing formation of the bacterial ubiquitin coat and activation of innate immunity. |
| Function | E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin. The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types. |
| Protein Name | Ranbp-Type And C3hc4-Type Zinc Finger-Containing Protein 1Hbv-Associated Factor 4Heme-Oxidized Irp2 Ubiquitin Ligase 1Hoil-1Hepatitis B Virus X-Associated Protein 4Ring Finger Protein 54Ring-Type E3 Ubiquitin Transferase Hoil-1Ubiquitin-Conjugating Enzyme 7-Interacting Protein 3 |
| Database Links | Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Ranbp-Type And C3hc4-Type Zinc Finger-Containing Protein 1 antibodyAnti-Hbv-Associated Factor 4 antibodyAnti-Heme-Oxidized Irp2 Ubiquitin Ligase 1 antibodyAnti-Hoil-1 antibodyAnti-Hepatitis B Virus X-Associated Protein 4 antibodyAnti-Ring Finger Protein 54 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Hoil-1 antibodyAnti-Ubiquitin-Conjugating Enzyme 7-Interacting Protein 3 antibodyAnti-RBCK1 antibodyAnti-C20orf18 antibodyAnti-RNF54 antibodyAnti-UBCE7IP3 antibodyAnti-XAP3 antibodyAnti-XAP4 antibody |
Information sourced from Uniprot.org