• Immunofluorescence analysis of U2OS cells using RASGRP3 antibody (STJ110101) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RASGRP3 antibody (STJ110101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RASGRP3 antibody (540-689) (STJ110101)

SKU:
STJ110101

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RASGRP3 (540-689) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RASGRP3
Gene ID: 25780
Uniprot ID: GRP3_HUMAN
Immunogen Region: 540-689
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 540-689 of human RASGRP3 (NP_056191.1).
Immunogen Sequence: VLACRRFARAPSLSSGHGSL PGSPSLPPAQDEVFEFPGVT AGHRDLDSRAITLVTGSSRK ISVRLQRATTSQATQTEPVW SEAGWGDSGSHTFPKMKSKF HDKAAKDKGFAKWENEKPRV HAGVDVVDRGTEFELDQDEG EETRQDGEDG
Function Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
Protein Name Ras Guanyl-Releasing Protein 3
Calcium And Dag-Regulated Guanine Nucleotide Exchange Factor Iii
Guanine Nucleotide Exchange Factor For Rap1
Database Links Reactome: R-HSA-1169092
Reactome: R-HSA-5673001
Alternative Antibody Names Anti-Ras Guanyl-Releasing Protein 3 antibody
Anti-Calcium And Dag-Regulated Guanine Nucleotide Exchange Factor Iii antibody
Anti-Guanine Nucleotide Exchange Factor For Rap1 antibody
Anti-RASGRP3 antibody
Anti-GRP3 antibody
Anti-KIAA0846 antibody

Information sourced from Uniprot.org

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