• Immunohistochemistry analysis of paraffin-embedded rat brain using RAPSN antibody (STJ28799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using RAPSN antibody (STJ28799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RAPSN Antibody (STJ28799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RAPSN antibody (1-353) (STJ28799)

SKU:
STJ28799

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAPSN (1-353) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAPSN
Gene ID: 5913
Uniprot ID: RAPSN_HUMAN
Immunogen Region: 1-353
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human RAPSN (NP_116034.2).
Immunogen Sequence: MGQDQTKQQIEKGLQLYQSN QTEKALQVWTKVLEKSSDLM GRFRVLGCLVTAHSEMGRYK EMLKFAVVQIDTARELEDAD FLLESYLNLARSNEKLCEFH KTISYCKTCLGLPGTRAGAQ LGGQVSLSMGNAFLGLSVFQ KALESFEKALRYAHNNDDAM LECRVCCSLGSFYAQVKDYE KALFFPCKAAELVNNYGKGW SLKYRAMSQYHMAVAYRLLG RLGSAMECCEESMKIALQH
Post Translational Modifications Ubiquitinated by the BCR(KLHL8) complex, leading to its degradation.
Function Postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin.
Protein Name 43 Kda Receptor-Associated Protein Of The Synapse
Rapsyn
43 Kda Postsynaptic Protein
Acetylcholine Receptor-Associated 43 Kda Protein
Ring Finger Protein 205
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Postsynaptic Cell Membrane
Cytoplasm
Cytoskeleton
Cytoplasmic Surface Of Postsynaptic Membranes
Alternative Antibody Names Anti-43 Kda Receptor-Associated Protein Of The Synapse antibody
Anti-Rapsyn antibody
Anti-43 Kda Postsynaptic Protein antibody
Anti-Acetylcholine Receptor-Associated 43 Kda Protein antibody
Anti-Ring Finger Protein 205 antibody
Anti-RAPSN antibody
Anti-RNF205 antibody

Information sourced from Uniprot.org

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