• Immunohistochemistry analysis of BRIX1 in paraffin-embedded human lung cancer using BRIX1 Rabbit polyclonal antibody (STJ116691) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BRIX1 Rabbit polyclonal antibody (STJ116691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BRIX1 antibody (1-353) (STJ116691)

SKU:
STJ116691

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRIX1 (1-353) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRIX1
Gene ID: 55299
Uniprot ID: BRX1_HUMAN
Immunogen Region: 1-353
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human BRIX1 (NP_060791.3).
Immunogen Sequence: MAATKRKRRGGFAVQAKKPK RNEIDAEPPAKRHATAEEVE EEERDRIPGPVCKGKWKNKE RILIFSSRGINFRTRHLMQD LRMLMPHSKADTKMDRKDKL FVINEVCEMKNCNKCIYFEA KKKQDLYMWLSNSPHGPSAK FLVQNIHTLAELKMTGNCLK GSRPLLSFDPAFDELPHYAL LKELLIQIFSTPRYHPKSQP FVDHVFTFTILDNRIWFRNF QIIEEDAALVEIGPRFVLN
Function Required for biogenesis of the 60S ribosomal subunit.
Protein Name Ribosome Biogenesis Protein Brx1 Homolog
Brix Domain-Containing Protein 2
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Ribosome Biogenesis Protein Brx1 Homolog antibody
Anti-Brix Domain-Containing Protein 2 antibody
Anti-BRIX1 antibody
Anti-BRIX antibody
Anti-BXDC2 antibody

Information sourced from Uniprot.org

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