Anti-RAPGEF3 antibody (1-200) (STJ25301)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAPGEF3 (1-200) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAPGEF3 |
| Gene ID | 10411 |
| Uniprot ID | RPGF3_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MVLRRMHRPRSCSYQLLLEH QRPSCIQGLRWTPLTNSEES LDFSESLEQASTERVLRAGR QLHRHLLATCPNLIRDRKYH LRLYRQCCSGRELVDGILAL GLGVHSRSQVVGICQVLLDE GALCHVKHDWAFQDRDAQFY RFPGPEPEPVGTHEMEEELA EAVALLSQRGPDALLTVALR KPPGQRTDEELDLIFEELLH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Epac1/Epac1/RAPGEF3 (NP_001092002.1). |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. |
| Function | Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 3Exchange Factor Directly Activated By Camp 1Exchange Protein Directly Activated By Camp 1Epac 1Rap1 Guanine-Nucleotide-Exchange Factor Directly Activated By CampCamp-Regulated Guanine Nucleotide Exchange Factor ICamp-Gefi |
| Database Links | Reactome: R-HSA-354192Reactome: R-HSA-381676Reactome: R-HSA-392517Reactome: R-HSA-422356 |
| Cellular Localisation | Endomembrane System |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 3 antibodyAnti-Exchange Factor Directly Activated By Camp 1 antibodyAnti-Exchange Protein Directly Activated By Camp 1 antibodyAnti-Epac 1 antibodyAnti-Rap1 Guanine-Nucleotide-Exchange Factor Directly Activated By Camp antibodyAnti-Camp-Regulated Guanine Nucleotide Exchange Factor I antibodyAnti-Camp-Gefi antibodyAnti-RAPGEF3 antibodyAnti-CGEF1 antibodyAnti-EPAC antibodyAnti-EPAC1 antibody |
Information sourced from Uniprot.org