• Immunofluorescence analysis of NIH-3T3 cells using Rap1GAP Rabbit monoclonal antibody (STJ11101912) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Rap1GAP Rabbit monoclonal antibody (STJ11101912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RAP1GAP antibody (1-100) [S2MR] (STJ11101912)

SKU:
STJ11101912

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RAP1GAP (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAP1GAP
Gene ID: 5909
Uniprot ID: RPGP1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rap1GAP (P47736).
Immunogen Sequence: MIEKMQGSRMDEQRCSFPPP LKTEEDYIPYPSVHEVLGRE GPFPLILLPQFGGYWIEGTN HEITSIPETEPLQSPTTKVK LECNPTARIYRKHFLGKEHF
Tissue Specificity Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
Function GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
Protein Name Rap1 Gtpase-Activating Protein 1
Rap1gap
Rap1gap1
Database Links Reactome: R-HSA-392517
Reactome: R-HSA-8853659
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Rap1 Gtpase-Activating Protein 1 antibody
Anti-Rap1gap antibody
Anti-Rap1gap1 antibody
Anti-RAP1GAP antibody
Anti-KIAA0474 antibody
Anti-RAP1GA1 antibody

Information sourced from Uniprot.org

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