Anti-RAP1B antibody (1-184) (STJ114791)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAP1B (1-184) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAP1B |
| Gene ID | 5908 |
| Uniprot ID | RAP1B_HUMAN |
| Immunogen Region | 1-184 |
| Immunogen Sequence | MREYKLVVLGSGGVGKSALT VQFVQGIFVEKYDPTIEDSY RKQVEVDAQQCMLEILDTAG TEQFTAMRDLYMKNGQGFAL VYSITAQSTFNDLQDLREQI LRVKDTDDVPMILVGNKCDL EDERVVGKEQGQNLARQWNN CAFLESSAKSKINVNEIFYD LVRQINRKTPVPGKARKKSS CQLL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human Rap1B (NP_056461.1). |
Additional Info
| Function | GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function. |
| Protein Name | Ras-Related Protein Rap-1bGtp-Binding Protein Smg P21b |
| Database Links | Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-392517Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8875555Reactome: R-HSA-8950505Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell MembraneCytoplasmCytosolCell JunctionMay Shuttle Between Plasma Membrane And CytosolPresence Of Krit1 And Cdh5 Is Required For Its Localization To The Cell Junction |
| Alternative Antibody Names | Anti-Ras-Related Protein Rap-1b antibodyAnti-Gtp-Binding Protein Smg P21b antibodyAnti-RAP1B antibodyAnti-OK antibodyAnti-SW-cl.11 antibody |
Information sourced from Uniprot.org