Anti-RAP1A antibody (39-138) (STJ25296)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAP1A (39-138) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAP1A |
| Gene ID | 5906 |
| Uniprot ID | RAP1A_HUMAN |
| Immunogen Region | 39-138 |
| Immunogen Sequence | SYRKQVEVDCQQCMLEILDT AGTEQFTAMRDLYMKNGQGF ALVYSITAQSTFNDLQDLRE QILRVKDTEDVPMILVGNKC DLEDERVVGKEQGQNLARQW |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 39-138 of human Rap1A (NP_002875.1). |
Additional Info
| Function | Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions. |
| Protein Name | Ras-Related Protein Rap-1aC21kgG-22kGtp-Binding Protein Smg P21aRas-Related Protein Krev-1 |
| Database Links | Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381676Reactome: R-HSA-392517Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8875555Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmPerinuclear RegionCell JunctionEarly EndosomeRecruited From Early Endosome To Late Endosome Compartment After Nerve Growth Factor (Ngf) StimulationLocalized With Rapgef2 At Cell-Cell JunctionsColocalized With Rapgef2 In The Perinuclear Region |
| Alternative Antibody Names | Anti-Ras-Related Protein Rap-1a antibodyAnti-C21kg antibodyAnti-G-22k antibodyAnti-Gtp-Binding Protein Smg P21a antibodyAnti-Ras-Related Protein Krev-1 antibodyAnti-RAP1A antibodyAnti-KREV1 antibody |
Information sourced from Uniprot.org