• Immunofluorescence analysis of U-2 OS cells using RALGDS Rabbit polyclonal antibody (STJ119942) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RALGDS Rabbit polyclonal antibody (STJ119942) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RALGDS in paraffin-embedded human thyroid cancer using RALGDS Rabbit polyclonal antibody (STJ119942) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RALGDS in paraffin-embedded rat brain using RALGDS Rabbit polyclonal antibody (STJ119942) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RALGDS in paraffin-embedded mouse testis using RALGDS Rabbit polyclonal antibody (STJ119942) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RALGDS Rabbit polyclonal antibody (STJ119942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RALGDS antibody (771-902) (STJ119942)

SKU:
STJ119942

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RALGDS (771-902) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RALGDS
Gene ID: 5900
Uniprot ID: GNDS_HUMAN
Immunogen Region: 771-902
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 771-902 of human RALGDS (NP_001258705).
Immunogen Sequence: CNSSSALPLYNQQVGDCCII RVSLDVDNGNMYKSILVTSQ DKAPAVIRKAMDKHNLEEEE PEDYELLQILSDDRKLKIPE NANVFYAMNSTANYDFVLKK RTFTKGVKVKHGASSTLPRM KQKGLKIAKGIF
Post Translational Modifications Phosphorylation of Tyr-814 by MET blocks HRAS binding.
Function Functions as a guanine nucleotide exchange factor (GEF) activating either RalA or RalB GTPases and plays an important role in intracellular transport. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. During bacterial clearance, recognizes 'Lys-33'-linked polyubiquitinated TRAF3 and subsequently mediates assembly of the exocyst complex.
Protein Name Ral Guanine Nucleotide Dissociation Stimulator
Ralgds
Ral Guanine Nucleotide Exchange Factor
Ralgef
Database Links Reactome: R-HSA-171007
Reactome: R-HSA-5673001
Cellular Localisation Cytoplasm
Nucleus
Localizes Mainly In The Peripheral Region Of The Cytoplasmic Membrane In Oocytes And In Preimplantation Embryos Until The 8-Cell Stage
Between The Late 1-Cell And The Early 2-Cell Stages
Nuclear Localization Becomes Stronger
After The 4-Cell Stage
Not Detected In The Nucleus
Alternative Antibody Names Anti-Ral Guanine Nucleotide Dissociation Stimulator antibody
Anti-Ralgds antibody
Anti-Ral Guanine Nucleotide Exchange Factor antibody
Anti-Ralgef antibody
Anti-RALGDS antibody
Anti-KIAA1308 antibody
Anti-RGF antibody

Information sourced from Uniprot.org

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