| Short Description : | Rabbit polyclonal RALGDS (771-902) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RALGDS |
| Gene ID: | 5900 |
| Uniprot ID: | GNDS_HUMAN |
| Immunogen Region: | 771-902 |
| Immunogen Sequence: | CNSSSALPLYNQQVGDCCII RVSLDVDNGNMYKSILVTSQ DKAPAVIRKAMDKHNLEEEE PEDYELLQILSDDRKLKIPE NANVFYAMNSTANYDFVLKK RTFTKGVKVKHGASSTLPRM KQKGLKIAKGIF |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 771-902 of human RALGDS (NP_001258705). |
| Post Translational Modifications | Phosphorylation of Tyr-814 by MET blocks HRAS binding. |
| Function | Functions as a guanine nucleotide exchange factor (GEF) activating either RalA or RalB GTPases and plays an important role in intracellular transport. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. During bacterial clearance, recognizes 'Lys-33'-linked polyubiquitinated TRAF3 and subsequently mediates assembly of the exocyst complex. |
| Protein Name | Ral Guanine Nucleotide Dissociation StimulatorRalgdsRal Guanine Nucleotide Exchange FactorRalgef |
| Database Links | Reactome: R-HSA-171007Reactome: R-HSA-5673001 |
| Cellular Localisation | CytoplasmNucleusLocalizes Mainly In The Peripheral Region Of The Cytoplasmic Membrane In Oocytes And In Preimplantation Embryos Until The 8-Cell StageBetween The Late 1-Cell And The Early 2-Cell StagesNuclear Localization Becomes StrongerAfter The 4-Cell StageNot Detected In The Nucleus |
| Alternative Antibody Names | Anti-Ral Guanine Nucleotide Dissociation Stimulator antibodyAnti-Ralgds antibodyAnti-Ral Guanine Nucleotide Exchange Factor antibodyAnti-Ralgef antibodyAnti-RALGDS antibodyAnti-KIAA1308 antibodyAnti-RGF antibody |
Information sourced from Uniprot.org
