Anti-RAG2 antibody (350-527aa aa) [4D5] (STJ98352)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAG-2 (350-527aa) expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-VDJ recombination-activating protein 2 (350-527aa aa) for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4D5 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAG2 |
| Gene ID | 5897 |
| Uniprot ID | RAG2_HUMAN |
| Immunogen | Purified recombinant fragment of human RAG-2 (350-527aa) expressed in E. Coli. |
| Immunogen Region | 350-527aa aa |
| Specificity | RAG-2 Monoclonal Antibody detects endogenous levels of RAG-2 protein. |
Additional Info
| Function | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3. |
| Protein Name | V(Dj Recombination-Activating Protein 2Rag-2 |
| Database Links | Reactome: R-HSA-1266695Reactome: R-HSA-5687128 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-V(Dj Recombination-Activating Protein 2 antibodyAnti-Rag-2 antibodyAnti-RAG2 antibody |
Information sourced from Uniprot.org