Anti-Rag A/B antibody (264-313 aa) (STJ95355)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RRAGA/B at the amino acid range 264-313
General Information
| Short Description | Rabbit polyclonal anti-Ras-related GTP-binding protein A and Ras-related GTP-binding protein B (264-313 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RRAGBRRAGA |
| Gene ID | 1032510670 |
| Uniprot ID | RRAGB_HUMANRRAGA_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RRAGA/B at the amino acid range 264-313 |
| Immunogen Region | 264-313 aa |
| Specificity | Rag A/B Polyclonal Antibody detects endogenous levels of Rag A/B protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation. |
| Function | Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. |
| Protein Name | Ras-Related Gtp-Binding Protein ARag ARagaAdenovirus E3 14.7 Kda-Interacting Protein 1Fip-1 |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-8866654Reactome: R-HSA-8943724Reactome: R-HSA-9639288 |
| Cellular Localisation | CytoplasmNucleusLysosomePredominantly CytoplasmicMay Shuttle Between The Cytoplasm And NucleusDepending On The Bound Nucleotide StateColocalizes In Vivo With Adenovirus E3-147k Mainly To The Cytoplasm Especially Near The Nuclear Membrane And In Discrete Foci On Or Near The Plasma Membrane |
| Alternative Antibody Names | Anti-Ras-Related Gtp-Binding Protein A antibodyAnti-Rag A antibodyAnti-Raga antibodyAnti-Adenovirus E3 14.7 Kda-Interacting Protein 1 antibodyAnti-Fip-1 antibodyAnti-RRAGA antibody |
Information sourced from Uniprot.org