• Immunofluorescence analysis of U-2 OS cells using Rad23A Rabbit monoclonal antibody (STJ11102274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Rad23A Rabbit monoclonal antibody (STJ11102274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Rad23A Rabbit monoclonal antibody (STJ11102274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using Rad23A Rabbit monoclonal antibody (STJ11102274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-RAD23A antibody (200-300) [S4MR] (STJ11102274)

SKU:
STJ11102274

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Rad23A (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD23A
Gene ID: 5886
Uniprot ID: RD23A_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Rad23A (P54725).
Immunogen Sequence: TGIPGSPEPEHGSVQESQVS EQPATEAAGENPLEFLRDQP QFQNMRQVIQQNPALLPALL QQLGQENPQLLQQISRHQEQ FIQMLNEPPGELADISDVEG E
Function Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
Protein Name Uv Excision Repair Protein Rad23 Homolog A
Hr23a
Hhr23a
Database Links Reactome: R-HSA-5689877
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Uv Excision Repair Protein Rad23 Homolog A antibody
Anti-Hr23a antibody
Anti-Hhr23a antibody
Anti-RAD23A antibody

Information sourced from Uniprot.org

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