• Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RAD1 antibody (STJ193200)

SKU:
STJ193200

€57.50 - €282.50
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cell cycle checkpoint protein RAD1 is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD1
Gene ID: 5810
Uniprot ID: RAD1_HUMAN
Specificity: This antibody detects endogenous levels of human RAD1
Immunogen: Synthesized peptide derived from the human RAD1
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds.endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths.and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Protein Name Cell Cycle Checkpoint Protein Rad1
Hrad1
Dna Repair Exonuclease Rad1 Homolog
Rad1-Like Dna Damage Checkpoint Protein
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cell Cycle Checkpoint Protein Rad1 antibody
Anti-Hrad1 antibody
Anti-Dna Repair Exonuclease Rad1 Homolog antibody
Anti-Rad1-Like Dna Damage Checkpoint Protein antibody
Anti-RAD1 antibody
Anti-REC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance