Anti-RACK1 antibody [R02-1K6] (STJA0034787)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human RACK1
General Information
| Short Description | Rabbit monoclonal anti-RACK1 for use in WB and IHC-P in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-1K6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RACK1 |
| Gene ID | 10399 |
| Uniprot ID | RACK1_HUMAN |
| Immunogen | A synthetic peptide of human RACK1 |
Additional Info
| Tissue Specificity | In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. |
| Post Translational Modifications | Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC. (Microbial infection) Phosphorylated by vaccinia virus B1 kinase on serine and threonine residues.this phosphorylation remodels the ribosome properties, favoring the viral mRNA translation. |
| Function | Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane. Promotes migration of breast carcinoma cells by binding to and activating RHOA. Acts as an adapter for the dephosphorylation and inactivation of AKT1 by promoting recruitment of PP2A phosphatase to AKT1. (Microbial infection) Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. (Microbial infection) Enhances phosphorylation of HIV-1 Nef by PKCs. (Microbial infection) In case of poxvirus infection, remodels the ribosomes so that they become optimal for the viral mRNAs (containing poly-A leaders) translation but not for host mRNAs. (Microbial infection) Contributes to the cap-independent internal ribosome entry site (IRES)-mediated translation by some RNA viruses. |
| Protein Name | Small Ribosomal Subunit Protein Rack1Cell Proliferation-Inducing Gene 21 ProteinGuanine Nucleotide-Binding Protein Subunit Beta-2-Like 1Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3Human Lung Cancer Oncogene 7 ProteinHlc-7Receptor For Activated C KinaseReceptor Of Activated Protein C Kinase 1 Cleaved Into - Small Ribosomal Subunit Protein Rack1 - N-Terminally ProcessedGuanine Nucleotide-Binding Protein Subunit Beta-2-Like 1 - N-Terminally ProcessedReceptor Of Activated Protein C Kinase 1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5626978 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmPerinuclear RegionNucleusPerikaryonCell ProjectionDendritePhagocytic CupRecruited To The Plasma Membrane Through Interaction With Krt1 Which Binds To Membrane-Bound Itgb1Also Associated With The Membrane In Oncogene-Transformed CellsPkc Activation Induces Translocation From The Perinuclear Region To The Cell PeripheryIn The BrainDetected Mainly In Cell Bodies And Dendrites With Little Expression In Axonal Fibers Or NucleiLocalized To Phagocytic Cups Following Infection By YPestis |
| Alternative Antibody Names | Anti-Small Ribosomal Subunit Protein Rack1 antibodyAnti-Cell Proliferation-Inducing Gene 21 Protein antibodyAnti-Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1 antibodyAnti-Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3 antibodyAnti-Human Lung Cancer Oncogene 7 Protein antibodyAnti-Hlc-7 antibodyAnti-Receptor For Activated C Kinase antibodyAnti-Receptor Of Activated Protein C Kinase 1 Cleaved Into - Small Ribosomal Subunit Protein Rack1 - N-Terminally Processed antibodyAnti-Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1 - N-Terminally Processed antibodyAnti-Receptor Of Activated Protein C Kinase 1 - N-Terminally Processed antibodyAnti-RACK1 antibodyAnti-GNB2L1 antibodyAnti-HLC7 antibodyAnti-PIG21 antibody |
Information sourced from Uniprot.org