Anti-RAC3 antibody (122-135 aa) [R01-6I8] (STJA0011871)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human RAC3
General Information
| Short Description | Rabbit monoclonal anti-Rac3 (122-135 aa) for use in WB and IHC-P in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-6I8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RAC3 |
| Gene ID | 5881 |
| Uniprot ID | RAC3_HUMAN |
| Immunogen | A synthetic peptide of human RAC3 |
| Immunogen Region | 122-135 aa |
Additional Info
| Tissue Specificity | Highest levels in brain, also detected in heart, placenta and pancreas. |
| Post Translational Modifications | (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. Ubiquitinated at Lys-166 in a FBXL19-mediated manner.leading to proteasomal degradation. |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. |
| Protein Name | Ras-Related C3 Botulinum Toxin Substrate 3P21-Rac3 |
| Database Links | Reactome: R-HSA-4086400Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmEndomembrane SystemCell ProjectionLamellipodiumPerinuclear RegionCell MembraneCytoskeletonMembrane-Associated When ActivatedColocalizes With Nrbp To Endomembranes And At The Cell Periphery In LamellipodiaColocalized With Cib1 In The Perinuclear Area And At The Cell Periphery |
| Alternative Antibody Names | Anti-Ras-Related C3 Botulinum Toxin Substrate 3 antibodyAnti-P21-Rac3 antibodyAnti-RAC3 antibody |
Information sourced from Uniprot.org