• Immunofluorescence analysis of L929 cells using RABIF Rabbit polyclonal antibody (STJ117284) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RABIF Rabbit polyclonal antibody (STJ117284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RABIF antibody (1-123) (STJ117284)

SKU:
STJ117284

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RABIF (1-123) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RABIF
Gene ID: 5877
Uniprot ID: MSS4_HUMAN
Immunogen Region: 1-123
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human RABIF (NP_002862.2).
Immunogen Sequence: MEPAEQPSELVSAEGRNRKA VLCQRCGSRVLQPGTALFSR RQLFLPSMRKKPALSDGSNP DGDLLQEHWLVEDMFIFENV GFTKDVGNIKFLVCADCEIG PIGWHCLDDKNSFYVALERV SHE
Tissue Specificity Ubiquitous.
Function Guanine-nucleotide-releasing protein that acts on members of the SEC4/YPT1/RAB subfamily. Stimulates GDP release from both YPT1, RAB3A and RAB10, but is less active on these proteins than on the SEC4 protein. Might play a general role in vesicular transport.
Protein Name Guanine Nucleotide Exchange Factor Mss4
Rab-Interacting Factor
Alternative Antibody Names Anti-Guanine Nucleotide Exchange Factor Mss4 antibody
Anti-Rab-Interacting Factor antibody
Anti-RABIF antibody
Anti-MSS4 antibody
Anti-RASGRF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance