• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RAB8A antibody (120-200 aa) (STJ191291)

SKU:
STJ191291

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ras-related protein Rab-8A (120-200 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB8A
Gene ID: 4218
Uniprot ID: RAB8A_HUMAN
Immunogen Region: 120-200 aa
Specificity: RAB8A Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from the human protein at the amino acid range 120-200
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Regulates the compacted morphology of the Golgi. Together with MYO5B and RAB11A participates in epithelial cell polarization. Also involved in membrane trafficking to the cilium and ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy.
Protein Name Ras-Related Protein Rab-8a
Oncogene C-Mel
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-2565942
Reactome: R-HSA-5620912
Reactome: R-HSA-5620916
Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus
Recycling Endosome Membrane
Cell Projection
Cilium
Cytoplasmic Vesicle
Phagosome
Phagosome Membrane
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cilium Basal Body
Midbody
Cilium Axoneme
Colocalizes With Optn At The Golgi Complex And In Vesicular Structures Close To The Plasma Membrane
In The Gdp-Bound Form
Present In The Perinuclear Region
Shows A Polarized Distribution To Distal Regions Of Cell Protrusions In The Gtp-Bound Form
Colocalizes With Pard3
Prkci
Exoc5
Ocln
Podxl And Rab11a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Lumenogenesis
Localizes To Tubular Recycling Endosome
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Non-Phosphorylated Rab8a Predominantly Localizes To The Cytoplasm Whereas Phosphorylated Rab8a Localizes To The Membrane
Alternative Antibody Names Anti-Ras-Related Protein Rab-8a antibody
Anti-Oncogene C-Mel antibody
Anti-RAB8A antibody
Anti-MEL antibody
Anti-RAB8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance