| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB8A, member RAS oncogene family (107aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:1000IHC-F 1:250-1:1500IHC-P 1:250-1:1500IF 1:50-1:250ELISA NDSchiweck J Murk K Ledderose J et al. Nature Communications 2021 PMID: 33674568Schiweck J Murk K Ledderose J et al. bioRxiv 2020 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
RAB8A |
| Gene ID: |
4218 |
| Uniprot ID: |
RAB8A_HUMAN |
| Immunogen Region: |
107aa C-Term |
| Accession Number: |
ENSG00000167461 |
| Specificity: |
Detects total Rab8 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab8a and GFP-Rab8b cds by Western blot. |
| Immunogen: |
Purified recombinant peptide derived from within residues 107 aa to the C-terminus of mouse Rab8a and Rab8b produced in E. coli. |
| Post Translational Modifications | Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Regulates the compacted morphology of the Golgi. Together with MYO5B and RAB11A participates in epithelial cell polarization. Also involved in membrane trafficking to the cilium and ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy. |
| Protein Name | Ras-Related Protein Rab-8aOncogene C-Mel |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-2565942Reactome: R-HSA-5620912Reactome: R-HSA-5620916Reactome: R-HSA-8854214Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideGolgi ApparatusRecycling Endosome MembraneCell ProjectionCiliumCytoplasmic VesiclePhagosomePhagosome MembraneCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyMidbodyCilium AxonemeColocalizes With Optn At The Golgi Complex And In Vesicular Structures Close To The Plasma MembraneIn The Gdp-Bound FormPresent In The Perinuclear RegionShows A Polarized Distribution To Distal Regions Of Cell Protrusions In The Gtp-Bound FormColocalizes With Pard3PrkciExoc5OclnPodxl And Rab11a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And LumenogenesisLocalizes To Tubular Recycling EndosomeRecruited To Phagosomes Containing SAureus Or MTuberculosisNon-Phosphorylated Rab8a Predominantly Localizes To The Cytoplasm Whereas Phosphorylated Rab8a Localizes To The Membrane |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-8a antibodyAnti-Oncogene C-Mel antibodyAnti-RAB8A antibodyAnti-MEL antibodyAnti-RAB8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
