| Host: | Goat |
| Applications: | WB/IHC-P/IHC-F |
| Reactivity: | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-RAB5C, member RAS oncogene family (118aa C-Term) for use in WB, IHC-P and IHC-F in Human, Mouse, Rat, Bovine, Canine, Chicken, Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep and Simian samples. Da |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:250-1:2000IHC-F 1:200-1:750IHC-P 1:200-1:750Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018 |
| Storage Instruction: | Store at-20 C for long-term storage. Store at 2-8 C for up to one month. |
| Gene Symbol: | RAB5C |
| Gene ID: | 5878 |
| Uniprot ID: | RAB5C_HUMAN |
| Immunogen Region: | 118aa C-Term |
| Accession Number: | ENSG00000108774 |
| Specificity: | Detects Rab5c by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab5c. This antibody is specific for Rab5c. It does not recognize Rab5a and Rab5b proteins. |
| Immunogen: | Purified recombinant peptide derived from within residues 118 aa to the C-terminus of mouse Rab5c produced in E. coli. |
| Immunogen Sequence: | ELQRQASPNIVIALAGNKAD LQRQASPNIVIALAGNKADL QRQASPNIVIALAGNKADLA RQASPNIVIALAGNKADLAS QASPNIVIALAGNKADLASK |
| Post Translational Modifications | Phosphorylation of Ser-85 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. |
| Protein Name | Ras-Related Protein Rab-5cL1880Rab5l |
| Database Links | Reactome: R-HSA-432722Reactome: R-HSA-6798695Reactome: R-HSA-8854214Reactome: R-HSA-8856828Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9820960 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideEarly Endosome MembraneMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-5c antibodyAnti-L1880 antibodyAnti-Rab5l antibodyAnti-RAB5C antibodyAnti-RABL antibody |
Information sourced from Uniprot.org
