Anti-RAB5B antibody (115aa C-Term) (STJ140061)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.
General Information
| Short Description | Goat polyclonal RAB5B, member RAS oncogene family (115aa C-Term) antibody for use in WB, IHC-P, IHC-F and IF in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian sam |
| Applications | WB/IHC-P/IHC-F/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 4 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:250-1:1000IF 1:50-1:250IHC-F 1:100-1:500IHC-P 1:100-1:500Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | RAB5B |
| Gene ID | 5869 |
| Uniprot ID | RAB5B_HUMAN |
| Accession Number | ENSG00000111540 |
| Immunogen | Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli. |
| Immunogen Region | 115aa C-Term |
| Immunogen Sequence | VKELQRQASPSIVIALAGNK KELQRQASPSIVIALAGNKA ELQRQASPSIVIALAGNKAD LQRQASPSIVIALAGNKADL QRQASPSIVIALAGNKADLA RQASPSIVIALAGNKADLAN |
| Specificity | Detects Rab5b protein by Western blot in cell lysates of transfected cells with GFP-Rab5b. This Ab is specific for Rab5b; it does not recognize Rab5a and Rab5c. |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. |
| Protein Name | Ras-Related Protein Rab-5b |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-8854214Reactome: R-HSA-8856828Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9820960 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideEarly Endosome MembraneMelanosomeEnriched In Stage I Melanosomes |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-5b antibodyAnti-RAB5B antibody |
Information sourced from Uniprot.org