Anti-RAB5A antibody (Internal Region) (STJ13101908)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the internal region of mouse Ras-related protein Rab-5A (RAB5A) conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in rat.
General Information
| Short Description | Rabbit polyclonal anti-RAB5A (Internal Region) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. Use at a dilution of 1:300 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Rab5a |
| Gene ID | 271457 |
| Uniprot ID | RAB5A_MOUSE |
| Immunogen | A synthetic peptide from the internal region of mouse Ras-related protein Rab-5A (RAB5A) conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in rat. |
| Immunogen Region | Internal Region |
| Specificity | Specific for RAB5A. |
Additional Info
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. |
| Protein Name | Ras-Related Protein Rab-5a |
| Database Links | Reactome: R-MMU-1660499Reactome: -MMU-8856828Reactome: -MMU-8873719Reactome: -MMU-8876198Reactome: -MMU-983231 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideEarly Endosome MembraneMelanosomeCytoplasmic VesicleCell ProjectionRuffleMembraneCytoplasmCytosolPhagosome MembraneEndosome MembraneEnriched In Stage I MelanosomesAlternates Between Membrane-Bound And Cytosolic Forms |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-5a antibodyAnti-Rab5a antibodyAnti-nnyRab5a antibody |
Information sourced from Uniprot.org