Anti-Rab5 antibody [R01-6G5] (STJA0034782)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Rab5
General Information
| Short Description | Rabbit monoclonal anti-Rab5 for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-6G5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RAB5A |
| Gene ID | 5868 |
| Uniprot ID | RAB5A_HUMAN |
| Immunogen | A synthetic peptide of human Rab5 |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. |
| Protein Name | Ras-Related Protein Rab-5a |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-8854214Reactome: R-HSA-8856828Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9636383Reactome: R-HSA-9820960Reactome: R-HSA-983231 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideEarly Endosome MembraneMelanosomeCytoplasmic VesicleCell ProjectionRuffleMembraneCytoplasmCytosolPhagosome MembraneEndosome MembraneEnriched In Stage I MelanosomesAlternates Between Membrane-Bound And Cytosolic Forms (Probable) |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-5a antibodyAnti-RAB5A antibodyAnti-RAB5 antibody |
Information sourced from Uniprot.org