• Anti-Rab3 antibody at 1:500 dilution; AtT-20 cell line lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-Rab3 antibody in B6 cells at 1:50 dilution; cells were fixed with methanol

Anti-RAB3A antibody (110aa C-Term) (STJ140058)

SKU:
STJ140058

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB3, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:250-1:1000
IHC-F 1:250-1:1500
IHC-P 1:250-1:1500
IF 1:50-1:250
1.Encarnação M Espada L Escrevente C et al. J Cell Biol. 2016 Jun. PMID: 27325790
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: RAB3A
Gene ID: 5864
Uniprot ID: RAB3A_HUMAN
Immunogen Region: 110aa C-Term
Accession Number: ENSG00000105649
Specificity: Detects total Rab3 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab3a, GFP-Rab3b, and GFP-Rab3c cds by Western blot.
Immunogen: Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab3a produced in E. coli.
Tissue Specificity Specifically expressed in brain.
Post Translational Modifications Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Function Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. Interacts with MADD (via uDENN domain).the GTP-bound form is preferred for interaction.
Protein Name Ras-Related Protein Rab-3a
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Reactome: R-HSA-888590
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasm
Cytosol
Lysosome
Cytoplasmic Vesicle
Secretory Vesicle
Cell Projection
Axon
Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Presynapse
Postsynapse
Cycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form
Alternative Antibody Names Anti-Ras-Related Protein Rab-3a antibody
Anti-RAB3A antibody

Information sourced from Uniprot.org

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