| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB3, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:1000IHC-F 1:250-1:1500IHC-P 1:250-1:1500IF 1:50-1:2501.Encarnação M Espada L Escrevente C et al. J Cell Biol. 2016 Jun. PMID: 27325790 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
RAB3A |
| Gene ID: |
5864 |
| Uniprot ID: |
RAB3A_HUMAN |
| Immunogen Region: |
110aa C-Term |
| Accession Number: |
ENSG00000105649 |
| Specificity: |
Detects total Rab3 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab3a, GFP-Rab3b, and GFP-Rab3c cds by Western blot. |
| Immunogen: |
Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab3a produced in E. coli. |
| Tissue Specificity | Specifically expressed in brain. |
| Post Translational Modifications | Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. |
| Function | Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. Interacts with MADD (via uDENN domain).the GTP-bound form is preferred for interaction. |
| Protein Name | Ras-Related Protein Rab-3a |
| Database Links | Reactome: R-HSA-181429Reactome: R-HSA-181430Reactome: R-HSA-210500Reactome: R-HSA-212676Reactome: R-HSA-264642Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-888590Reactome: R-HSA-9662360 |
| Cellular Localisation | CytoplasmCytosolLysosomeCytoplasmic VesicleSecretory VesicleCell ProjectionAxonCell MembraneLipid-AnchorCytoplasmic SidePresynapsePostsynapseCycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-3a antibodyAnti-RAB3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
