Anti-RAB14 antibody (110aa C-Term) (STJ140071)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab14 produced in E. coli.
STJ140071
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General Information

Short DescriptionGoat polyclonal RAB14, member RAS oncogene family (110aa C-Term) antibody for use in WB and IF in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datashee
ApplicationsWB/IF
HostGoat
ReactivityHuman/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration2 mg/mL
PurificationThis antibody is epitope-affinity purified from goat antiserum.
Dilution RangeWB 1:250-1:2000
IF 1:25-1:200
FormulationPBS, 20% Glycerol and 0.05% Sodium Azide.
Storage InstructionFor continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Information

Gene SymbolRAB14
Gene ID51552
Uniprot IDRAB14_HUMAN
Accession NumberENSG00000119396
ImmunogenPurified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab14 produced in E. coli.
Immunogen Region110aa C-Term
Immunogen SequenceMTDARNLTNPNTVIILIGNK TDARNLTNPNTVIILIGNKA DARNLTNPNTVIILIGNKAD ARNLTNPNTVIILIGNKADL RNLTNPNTVIILIGNKADLE NLTNPNTVIILIGNKADLEA
SpecificityDetects Rab14 by Western blot in transfected cells with GFP-Rab14.

Additional Info

Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes. Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion. Mediates endosomal tethering and fusion through the interaction with RUFY1 and RAB4B. Interaction with RAB11FIP1 may function in the process of neurite formation.
Protein Name Ras-Related Protein Rab-14
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1660499
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Recycling Endosome
Early Endosome Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Cytoplasmic Vesicle
Phagosome
Recruited To Recycling Endosomes By Dennd6a
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Colocalizes With Rab11fip1 On Punctate Vesicles
Alternative Antibody Names Anti-Ras-Related Protein Rab-14 antibody
Anti-RAB14 antibody

Information sourced from Uniprot.org

Citations

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