Anti-RAB2A antibody (110aa C-Term) (STJ140057)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab2b produced in E. coli.
General Information
| Short Description | Goat polyclonal RAB2, member RAS oncogene family (110aa C-Term) antibody for use in WB in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datasheet includ |
| Applications | WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:250-1:2000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | RAB2A |
| Gene ID | 5862 |
| Uniprot ID | RAB2A_HUMAN |
| Accession Number | ENSG00000104388 |
| Immunogen | Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab2b produced in E. coli. |
| Immunogen Region | 110aa C-Term |
| Specificity | Detects total levels of Rab2 protein in transfected cells with GFP-Rab2a and GFP-Rab2b by Western blot. |
Additional Info
| Post Translational Modifications | Prenylated. Prenylation is required for association with cellular membranes. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. RAB2A regulates autophagy by promoting autophagosome-lysosome fusion via recruitment of the HOPS endosomal tethering complex.this process involves autophagosomal RAB2A and lysosomal RAB39A recruitment of HOPS subcomplexes VPS39-VPS11 and VPS41-VPS16-VPS18-VPS33A, respectively, which assemble into a functional complex to mediate membrane tethering and SNAREs-driven membrane fusion. Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi. Together with RAB2B, redundantly required for efficient autophagic flux. |
| Protein Name | Ras-Related Protein Rab-2a |
| Database Links | Reactome: R-HSA-162658Reactome: R-HSA-8873719 |
| Cellular Localisation | Endoplasmic Reticulum-Golgi Intermediate Compartment MembraneLipid-AnchorCytoplasmic SideMelanosomeEndoplasmic Reticulum MembraneGolgi Apparatus MembraneCytoplasmic VesicleSecretory VesicleAcrosomeAutophagosome MembraneLocalized In The Golgi Apparatus In The Round Spermatids And In The Acrosome In The Elongating SpermatidIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-2a antibodyAnti-RAB2A antibodyAnti-RAB2 antibody |
Information sourced from Uniprot.org