• Anti-Rab35 antibody at 1:1000 dilution; 293HEK transfected with GFP-Rab35; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB35 antibody (110aa C-Term) (STJ140106)

SKU:
STJ140106

Current Stock:
Host: Goat
Applications: WB
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RA35, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 4 mg/ml
Dilution Range: WB 1:250-1:2000
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: RAB35
Gene ID: 11021
Uniprot ID: RAB35_HUMAN
Immunogen Region: 110aa C-Term
Accession Number: ENSG00000111737
Specificity: Detects Rab35 in cell lysates and transfected cells by Western blot.
Immunogen: Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab35 produced in E. coli.
Post Translational Modifications AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB35 constitutively active. Phosphocholinated by L.pneumophila AnkX. Both GDP-bound and GTP-bound forms can be phosphocholinated. Phosphocholination inhibits the GEF activity of DENND1A.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
Protein Name Ras-Related Protein Rab-35
Gtp-Binding Protein Ray
Ras-Related Protein Rab-1c
Database Links Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Membrane
Clathrin-Coated Pit
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Endosome
Melanosome
Present On Sorting Endosomes And Recycling Endosome Tubules
Tends To Be Enriched In Pip2-Positive Cell Membrane Domains
During Mitosis
Associated With The Plasma Membrane And Present At The Ingressing Furrow During Early Cytokinesis As Well As At The Intercellular Bridge Later During Cytokinesis
Identified In Stage I To Stage Iv Melanosomes
Alternative Antibody Names Anti-Ras-Related Protein Rab-35 antibody
Anti-Gtp-Binding Protein Ray antibody
Anti-Ras-Related Protein Rab-1c antibody
Anti-RAB35 antibody
Anti-RAB1C antibody
Anti-RAY antibody

Information sourced from Uniprot.org

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