Anti-RAB35 antibody (99-148 aa) (STJ95296)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RAB35 at the amino acid range 99-148
General Information
| Short Description | Rabbit polyclonal anti-Ras-related protein Rab-35 (99-148 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB35 |
| Gene ID | 11021 |
| Uniprot ID | RAB35_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RAB35 at the amino acid range 99-148 |
| Immunogen Region | 99-148 aa |
| Specificity | Rab 35 Polyclonal Antibody detects endogenous levels of Rab 35 protein. |
Additional Info
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB35 is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes. |
| Protein Name | Ras-Related Protein Rab-35Gtp-Binding Protein RayRas-Related Protein Rab-1c |
| Database Links | Reactome: R-HSA-8854214Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideMembraneClathrin-Coated PitCytoplasmic VesicleClathrin-Coated VesicleEndosomeMelanosomePresent On Sorting Endosomes And Recycling Endosome TubulesTends To Be Enriched In Pip2-Positive Cell Membrane DomainsDuring MitosisAssociated With The Plasma Membrane And Present At The Ingressing Furrow During Early Cytokinesis As Well As At The Intercellular Bridge Later During CytokinesisIdentified In Stage I To Stage Iv MelanosomesColocalizes With Acap2 And Rusc2 At The Membrane Protrusions Of Hek293t Cells |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-35 antibodyAnti-Gtp-Binding Protein Ray antibodyAnti-Ras-Related Protein Rab-1c antibodyAnti-RAB35 antibodyAnti-RAB1C antibodyAnti-RAY antibody |
Information sourced from Uniprot.org