• Immunofluorescence analysis of A549 cells using RAB31 antibody (STJ116210). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RAB31 antibody (STJ116210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RAB31 antibody (1-195) (STJ116210)

SKU:
STJ116210

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB31 (1-195) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB31
Gene ID: 11031
Uniprot ID: RAB31_HUMAN
Immunogen Region: 1-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human RAB31 (NP_006859.2).
Immunogen Sequence: MMAIRELKVCLLGDTGVGKS SIVCRFVQDHFDHNISPTIG ASFMTKTVPCGNELHKFLIW DTAGQERFHSLAPMYYRGSA AAVIVYDITKQDSFYTLKKW VKELKEHGPENIVMAIAGNK CDLSDIREVPLKDAKEYAES IGAIVVETSAKNAINIEELF QGISRQIPPLDPHENGNNGT IKVEKPTMQASRRCC
Tissue Specificity Highest expression in placenta and brain with lower levels in heart and lung. Not detected in liver, skeletal muscle, kidney or pancreas.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
Protein Name Ras-Related Protein Rab-31
Ras-Related Protein Rab-22b
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Golgi Apparatus
Trans-Golgi Network
Trans-Golgi Network Membrane
Lipid-Anchor
Cytoplasmic Side
Early Endosome
Cytoplasmic Vesicle
Phagosome
Phagosome Membrane
Rapidly Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Alternative Antibody Names Anti-Ras-Related Protein Rab-31 antibody
Anti-Ras-Related Protein Rab-22b antibody
Anti-RAB31 antibody
Anti-RAB22B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance