Anti-Rab27A antibody [R06-1F4] (STJA0036901)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human RAB27A
STJA0036901
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General Information

Short DescriptionRabbit monoclonal anti-Rab27A for use in WB, ICC, IF, IP and FC in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF/IP/FC
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR06-1F4
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
IP 1:50
FC 1:50-1:100
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolRAB27A
Gene ID5873
Uniprot IDRB27A_HUMAN
ImmunogenA synthesized peptide derived from human RAB27A

Additional Info

Tissue Specificity Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Protein Name Ras-Related Protein Rab-27a
Rab-27
Gtp-Binding Protein Ram
Database Links Reactome: R-HSA-264876
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Reactome: R-HSA-9824585
Cellular Localisation Membrane
Lipid-Anchor
Melanosome
Late Endosome
Lysosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To Endosomal Exocytic Vesicles
Alternative Antibody Names Anti-Ras-Related Protein Rab-27a antibody
Anti-Rab-27 antibody
Anti-Gtp-Binding Protein Ram antibody
Anti-RAB27A antibody
Anti-RAB27 antibody

Information sourced from Uniprot.org

Citations

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