| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB27A, member RAS oncogene family (120aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
2 mg/ml |
| Dilution Range: |
WB 1:250-1:2000IF 1:50-1:200IHC-P 1:50-1:400IHC-F 1:50-1:400Ramsay E Ravina M Sarkhel S et al. Pharmaceutics 2020 Jul PMID: 32708811Domingues L Hurbain I Gilles-Marsens F et al. Nat Commun 2020 Jun PMID: 32532976Domingue |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
RAB27A |
| Gene ID: |
5873 |
| Uniprot ID: |
RB27A_HUMAN |
| Immunogen Region: |
120aa C-Term |
| Accession Number: |
ENSG00000069974 |
| Specificity: |
Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody is specific for Rab27a. It does not recognize Rab27b protein. |
| Immunogen: |
Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli. |
| Tissue Specificity | Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
| Protein Name | Ras-Related Protein Rab-27aRab-27Gtp-Binding Protein Ram |
| Database Links | Reactome: R-HSA-264876Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | MembraneLipid-AnchorMelanosomeLate EndosomeLysosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To Endosomal Exocytic Vesicles |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-27a antibodyAnti-Rab-27 antibodyAnti-Gtp-Binding Protein Ram antibodyAnti-RAB27A antibodyAnti-RAB27 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
