Anti-RAB21 antibody (1-225) (STJ113990)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAB21 (1-225) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB21 |
| Gene ID | 23011 |
| Uniprot ID | RAB21_HUMAN |
| Immunogen Region | 1-225 |
| Immunogen Sequence | MAAAGGGGGGAAAAGRAYSF KVVLLGEGCVGKTSLVLRYC ENKFNDKHITTLQASFLTKK LNIGGKRVNLAIWDTAGQER FHALGPIYYRDSNGAILVYD ITDEDSFQKVKNWVKELRKM LGNEICLCIVGNKIDLEKER HVSIQEAESYAESVGAKHYH TSAKQNKGIEELFLDLCKRM IETAQVDERAKGNGSSQPGT ARRGVQIIDDEPQAQTSGGG CCSSG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human RAB21 (NP_055814.1). |
Additional Info
| Tissue Specificity | Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB21 is involved in membrane trafficking control. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. Involved in neurite growth. Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion. Modulates protein levels of the cargo receptors TMED2 and TMED10, and required for appropriate Golgi localization of TMED10. |
| Protein Name | Ras-Related Protein Rab-21 |
| Database Links | Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Endoplasmic Reticulum MembraneLipid-AnchorGolgi ApparatusTrans-Golgi NetworkGolgi Apparatus MembraneEarly Endosome MembraneCytoplasmic Vesicle MembraneCleavage FurrowCell ProjectionNeuron ProjectionColocalizes With Ankrd27 And Vamp7 In NeuritesIn Nonpolarized Epithelial Caco-2 CellsFound In The Endoplasmic ReticulumIn Polarized CellsObserved In Vesicles In The Apical CytoplasmDuring MitosisIn Mid-TelophaseLocalized In The Ingressing Cleavage FurrowIn Late TelophaseDetected At The Opposite Poles Of The Daughter CellsIn Vesicles At The Base Of Lamellipodia Formed By The Separating Daughter Cells |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-21 antibodyAnti-RAB21 antibodyAnti-KIAA0118 antibody |
Information sourced from Uniprot.org